Journal: European Heart Journal

Article Title: Left bundle branch vs biventricular pacing: mechanistic insights from a canine model

doi: 10.1093/eurheartj/ehaf1093

Figure Lengend Snippet: Molecular targets in cardiac remodeling by BiVP and LBBP. Western blotting results of BNP, TGF-β, KCNIP2, SERCA2a, Cx43, ANKRD1 protein expression (A, C, E) in septum and LV lateral walls from NC, DHF, and BiVP and LBBP groups, and relative protein expression (B, D, F). Represented images and statistical data (G) of cardiac fibrosis detected using Masson's staining. † P < .05 between septal and lateral walls. Abbreviations as in

Article Snippet: The levels of several key proteins were examined using western blot, including ANKRD1 (Proteintech, 11427-1-AP), BNP (Abclonal, A23996), Cx43 (Proteintech, 26980-1-AP), GSNOR (Proteintech, 66193-1-Ig), GLUD1 (Proteintech, 14299-1-AP), KCNIP2 (Abclonal, A7100), SERCA2a (Abclonal, A0098), TGF-β (Proteintech, 81746-2-RR), and GAPDH (Proteintech, 10494-1-AP).

Techniques: Western Blot, Expressing, Staining

Journal: European Heart Journal

Article Title: Left bundle branch vs biventricular pacing: mechanistic insights from a canine model

doi: 10.1093/eurheartj/ehaf1093

Figure Lengend Snippet: Overview of the key findings. This study compares the treatment efficacy of LBBP and BiVP, and relating electrocardiographic, echocardiographic and cellular (reverse) remodeling in a canine DHF model. The results show that LBBP restores electrical synchrony (QRSd) to a similar extent as BiVP, while leads to larger LV-GLS improvement than BiVP. Both LBBP and BiVP reverse the protein level of KCNIP2, SERCA2a and Cx43. Moreover, LBBP reverses abnormalities in cytoskeleton-associated proteins, TGF-β signaling, and SERCA2a expression more than BiVP, as well as enhances myocardial energy metabolism via more efficient TCA cycling and ATP production. ANKRD1, ankyrin repeat domain 1; ATP, adenosine triphosphate; BiVP, biventricular pacing; BNP, B-type natriuretic peptide; Cx43, connexin 43; DHF dyssynchronous heart failure; ECG, electrocardiogram; echo, echocardiogram; GLUD1, glutamate dehydrogenase 1; GSNOR S-nitrosoglutathione reductase; KCNIP2, potassium voltage-gated channel interacting protein 2; LBBP, left bundle branch pacing; LV-GLS, left ventricular global longitudinal strain; NC, normal control; SERCA2a, sarcoplasmic/endoplasmic reticulum Ca 2+ ATPase 2a; TCA, tricarboxylic acid; TGF-β, Transforming growth factor-β.

Article Snippet: The levels of several key proteins were examined using western blot, including ANKRD1 (Proteintech, 11427-1-AP), BNP (Abclonal, A23996), Cx43 (Proteintech, 26980-1-AP), GSNOR (Proteintech, 66193-1-Ig), GLUD1 (Proteintech, 14299-1-AP), KCNIP2 (Abclonal, A7100), SERCA2a (Abclonal, A0098), TGF-β (Proteintech, 81746-2-RR), and GAPDH (Proteintech, 10494-1-AP).

Techniques: Expressing, Control

Journal: eBioMedicine

Article Title: Neural synaptic vesicle autoimmunity following aerosolized porcine neural tissue exposure: insights into autoimmune inflammatory polyradiculoneuropathy

doi: 10.1016/j.ebiom.2025.106053

Figure Lengend Snippet: Confirmation of synaptophysin- and GAP43-IgG in patients with OIPN by ELISA and CBA . a) Fifteen of 20 patients with OIPN sera tested positive for synaptophysin-IgG by ELISA compared to none of the control cohorts. b) Representative immunofluorescent images of IgG binding to GFP-tagged synaptophysin-transfected COS7 cells. Commercial synaptophysin-IgG and OIPN-IgG (magenta) co-localises (white in merge) with GFP-tagged synaptophysin protein (green). c) Twelve of 20 sera from patients with OIPN tested positive for GAP43-IgG by ELISA. d) Representative immunofluorescent images of IgG binding (magenta) to GFP-tagged GAP43 transfected COS7 cells. Colocalisation (white in merge) is observed for commercial antibody and OIPN-IgG with GFP-GAP43 protein. b and d) DNA is labelled with DAPI, blue. Healthy adult human IgG fails to bind to transfected cells. Note: No IgG bound to non-transfected cells (solitary blue nuclei). Scale bars, 20 μm. Key: OIPN, occupational inflammatory polyradiculoneuropathy; ELISA, enzyme-linked immunosorbent assay; GFP, green fluorescence protein; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Immulon 2HB plates were coated (10 ng/well) with PhIP-Seq-identified antigens: synaptophysin peptide 224-313 (Mayo Clinic Proteomics Core Facility) or GAP43 recombinant protein (Novus, #NBP2-53033) diluted in 0.01 M PBS, pH 7.4.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Binding Assay, Transfection, Fluorescence